Transcript levels were quantitated using two separate standard curves for the 18S rRNA reference and the ACHE transcript (using sample oACHE-S-24B) and normalized (ACHE value/18S value).
The language formerly known as Serbo-Croatian has split into three separate standard languages: Bosnian, Croatian, and Serbian (BCS).
We calculated sensitivity, specificity, positive predictive value, and negative predictive value of each of the sputum NAATs in reference to two separate gold standards.
Second, our design, employing two separate internal standards, enabled us to contrast within-decade and between-decade comparisons even more directly than it had been possible in the study by Hoeckner et al., [ 17].
Importantly, our design with two separate internal standards enabled us to directly contrast within-decade and between-decade comparisons for the same distance (see the grey boxes in Figure 2A C).
Like its older, rules-and-principles cousin, the law is a hybrid, a creature of the two separate sets of standards, one grounded in social facts, the other in moral facts, that interpretation blends together (cf. Raz 1986).
The solutions were swirled for 10 min and filtered through Whatmann No. 40 filter paper into two separate 100-mL standard flasks which was then diluted to the mark with respective solvents.
Each 96-well plate contained a no template negative control and two separate 5-point standard curves ranging from 0.024 to 15 ng using leukocyte DNA.
The quantification was performed in two separate experiments with separately prepared standard curves but with the same batch of samples.
