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τ′ was calculated from τ, the angle between the line that connects two segregated daughter nuclei and the tangential line crossing the mother nucleus on the projection plane view (see Materials and Methods), which was measured as the angle between the division plane and the radial line (left two panels of Fig. 4A).
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They found two segregating sites (see table 1), together characterising two haplotypes (Y1 and Y2).
After initial molecular sexing, two segregating Y-SNPs were identified in 13 bulls.
Based on available DNA sequence from homozygotes of the two segregating haplotypes, a PCR based diagnostic assay was designed to detect 2 segregating indels within APL1A.
We expect to detect two segregating loci: the brown locus (Tyrp1) on Chr. 4 at 80Mb and the dilute locus (Myo5a) on Chr. 9 at 155 Mb.
Consider a single locus with two segregating alleles.
QTL mapping was successfully applied based on two segregating rye populations.
Two significant effects for female fertility QTL were identified; near the centromere with five segregating sire families, and near the end of the chromosome with two segregating sire families.
These NILs were backcrossed to B73 and their progeny were selfed to produce two segregating NIL families of ∼3500 plants.
We report the first genome-wide QTL analysis across two segregating rye populations for a comprehensive set of agronomic traits.
Genomic DNA was extracted from leaf samples of the two segregating populations at seedling stage using the procedure described by [ 18].
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