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Filamentous phage-displaying scFvs were selected against nmAb-KT and were enriched through five rounds of modified subtractive panning.
Our expert panel reached consensus on a final list of research priorities through an in-person meeting (phase 3) and two rounds of a modified Delphi process (phases 2 and 4).
After three rounds of the modified Delphi process, five variables were selected by the combination of the BoA decisions and the statistics of the VHR.
There were two rounds of review based on this modified Delphi methodology.
For the modified T7 method, after two rounds of amplification the product measured a mean of 3400 bases on the Agilent Bioanalyzer PicoChip.
*1 array discarded due to poor hybridization Total RNA from the BT474 breast cancer cell line and StratRef was linearly amplified through two rounds of in vitro transcription (IVT) based on a modified T7 amplification[ 19].
The desulphonated bisulphite modified genomic DNA was amplified by two rounds of PCR using double nested oligonucleotide primers in a 59 50°C touchdown PCR protocol.
MEIOSIS = a modified type of cell division in sexually reproducing organisms consisting of two rounds of cell division but only one round of DNA replication.
At all stages (i.e. desktop research and interviews with experts; two rounds of Delphi study; a validation workshop) the experts consulted were divided on the final issue: whether or not a new or modified standard is needed.
At all stages i.e. desktop research, interviews with experts, two rounds of Delphi study, and validation workshop, the experts consulted were divided on the final issue i.e. whether or not a new or modified standard is needed.
Two rounds of simulation results are reported including the first-round blind test and the second-round modeling refinement.
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