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Because the library is compact and a protease sensitive helper phage, KM13 [ 37] was used during bio-panning, only one or two round of selection were performed on eight different antigens.
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Transfected cells were put through two rounds of selection using appropriate antibiotic (puromycin 2 μg/ml, G418 400 μg/ml) to select multiple stable clones.
Finally, two rounds of selection and amplification in a biopanning procedure against immobilized endoxylanase inhibitor were performed.
Human κ constant region cDNA was selected from a N-terminal DBD fusion lymphocyte cDNA library after two rounds of selection with anti-κ antibody.
Sequence analysis showed enrichment of several clones after two rounds of selection compared to round 1, functionally validating our novel selection method.
Briefly, polyadenylated RNA was isolated from VACV-infected S2 cells by two rounds of selection using the Dynabeads mRNA Direct Kit (Invitrogen).
Between one and two rounds of selection were performed for each antigen.
Poly(A)+ RNA was purified by two rounds of selection using the Poly(A Pure kit (Ambion, Inc).
After two rounds of selection, FP was significantly more pronounced in the HFP line than the LFP line.
After only two rounds of selection, five candidate sequences specific for BoNT/A Hc-peptiod had K d values ranging from 1.09 μM to 4.20 μM.
Proliferated calli were subjected to two rounds of selection containing 8 mg/l and 10 mg/l phosphinothricin for four weeks [ 23, 12].
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