In this note, we show that there are no algorithms of running time O⁎ 2ck) and O⁎ 2cklog2k) for the two restrictions of WSP, respectively, with any c<1, unless the Strong Exponential Time Hypothesis fails.
Clade 4 displayed two restrictions sites for SspI whereas the three remaining clades have three sites.
This in silico approach revealed the necessity to use a combination of two restriction enzymes for T-RFLP analysis of the Chrysophyceae.
Among the four clades observed, clade 3 displayed two restriction sites for the RsaI enzyme whereas the three remaining clades only had one.
The T-DNA of the vector contained the bar selection gene under the control of the Ubi1 intron promoter and two restriction sites for cloning the RNAi construct, separated by a rice waxy intron and driven by the cauliflower mosaic virus 35S promoter.
In Table 1, I explore the impact of the latter two restrictions by comparing summary statistics for the full sample with the sample matched to birth data and the sibling-pair sample.
For restriction enzyme digestion analysis of mutations, two restriction enzyme reactions for each PCR product were set up: in one reaction, the corresponding restriction enzyme was added; in the other reaction, the enzyme was replaced by water as a negative control.
These two marker transgenes were flanked with loxP sites and two restriction enzyme sites for convenient insertion of homology arms of interest.
Two restriction sites were created for those mutations in each segment, Fsp I and Dra I, respectively.
For the plasmid utilized in the study, there is a single EcoRI restriction site (simply linearizes the plasmid) while two restriction sites are present for PstI (generating two fragments).
