It combines NOE and RDC data, it relies on unambiguous H N − H N NOEs and two RDCs per residue of the spin pairs N− C′, N− Cα.
The results indicate that the model is robust and accurate in reproducing the behaviour of the two RDCs, especially in what refers to Tf, whose estimates were very close to the observations.
Methods: Thirty-four RDC bipolar I manic, 17 psychotic unipolar depressed, and 72 nonpsychotic unipolar depressed patients were assessed at index hospitalization and prospectively followed-up at 2, 4.5, 7.5 and 10 years.
Mice had been sacrificed and the uptake of this antigen by the four RDC subsets was evaluated by flow cytometry.
These five RDCs also did not fit those generated from the X-ray crystal structure and from the MD-generated molecular ensembles.
Eight RDCs of ring B, which were not used as structural restraints during MD, were then back calculated using MD structures.
DTx administration resulted in the depletion of these two CD11chi RDC subsets as well as MoRDC from the lungs of the DTR mice and also partial depletion of LN DC from the MLN (not depicted).
The above findings implicated one or both of the two migrant RDC subsets found in the MLN after respiratory virus infection as critical for the induction of anti-viral T cell responses presumably through their capacity to transport the viral antigen to the draining MLN and possibly to directly serve as APC for naïve T cells.
Two other RDCs, for residues 118 and 184, were removed from the analysis because their values were found to lie significantly outside the alignment tensor window.
However, two subsets of RDC, the CD103+ RDC and CD11bhi RDC were also potential candidates in antigen uptake and recognition because of their preferential localization to the airway mucosa of epithelium and the lung submucosa/parenchyma, respectively [19].
