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We arranged U6 promoter driving shRNA expression and UbiC promoter in two promoter arrangements.
Two promoter libraries were simultaneously introduced in front of two target genes, enabling orthogonal expression as demonstrated by principal component analysis.
To determine the APO1 allele from 12 recipients, we sequenced two promoter regions and the third exon (Fig. 3a), which showed some DNA variations between genotypes of mapping bi-parents (Habataki and Koshihikari) (Ookawa et al. 2010).
Of two promoter systems evaluated, the one which had been designed to be less sensitive to copper generally was more effective at achieving sterilization and more responsive to repression.
The results revealed that the two promoter regions have different regulatory element sites (Fig 4D).
To harvest promoters that were differentially regulated inside red ripe Campari tomatoes, two promoter probe-gfp libraries were used [50].
Similar(6)
To identify promoters that are strongly regulated in Salmonella during colonization of tomato fruits, two promoter-gfp libraries were screened and tomato-specific constructs were isolated.
Despite the detailed understanding of this complex two-promoter system, the purpose of having two promoters for a single operon remains unclear.
Of these, 516 loci were differently methylated in at least two promoter-proximal CpG sites over two discrete Csp6I fragments.
Arabidopsis Col-0 plants were transformed with Agrobacterium tumefaciens GV3101 strain harboring either one of the two promoter-pKGWFS7 plasmids using a standard floral dipping method [ 48].
To analyze the efficacy of this method, we tested several concentration ranges for TXNIP silencing using two promoter-targeted siRNAs together in rat retinal ECs in culture.
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