To determine whether any of these species represent phosphorylated forms, we treated protein extracts with calf intestinal phosphatase (CIP) before analysis by 2D PAGE. Figure 1B and Figure 2 indicate that two prominent acidic forms (A,B) are lost following CIP treatment, consistent with them being phosphorylated forms.
Consistent with these findings, Rb was indeed phosphorylated on serine 780 in early-G1 in CHO cells, and there were two prominent phosphorylated forms of Rb that contained this ser780P residue (Fig. 4A, TCE, G0-6 hrs, lower two arrows).
The institutional view holds that the purpose of the institution defines its obligations, taking precedence over spouses' desires, either, in the two most prominent forms, in the service of a procreative union, or to protect spousal love.
This entry will consider the four most prominent forms of skeptical theism.
In an effort to defeat the Brownback bill, two prominent Hollywood couples formed an organization they called Cures Now.
Aries has three prominent stars forming an asterism, designated Alpha, Beta, and Gamma Arietis by Johann Bayer.
This asymmetry takes the form of two prominent peaks, suggesting two prominent ordering length scales.
In 1915 Ince formed Triangle Pictures Production Company in partnership with two prominent directors, Mack Sennett and D.W. Griffith.
Two prominent Republicans, Romney and former Minnesota Governor Tim Pawlenty, have jumped in the race by forming exploratory committees to begin raising money.
