The in silico model suggested four other pathways of citrate oxidation that could also produce acetate and/or succinate under acceptor limiting conditions.
In addition we have identified elements of two other pathways, the dopamine receptor signaling pathway (GO 0007212, adjusted P <0.001) and the steroid hormone mediated signaling pathway (GO 0043401, adjusted P <0.01).
Recent studies have implicated two other pathways that contribute to the regulation of accurate chromosome segregation: Chk1/Wat1-dependent regulation of the mitotic spindle microtubule structure (Verma et al. 2014) and histone variant H2A.Z-dependent regulation of cohesin dynamics.
To investigate whether concurrent inhibition of two other pathway components could improve the efficiency of Rapamycin, cells were concomitantly treated with ZSTK474 and Rapamycin.
In ER+, PI3K mutant breast cancer cells, this negative edge dampens (but does not block) ER signaling, and all four other pathways are active; yielding the collective effect of a significant proliferation propensity and lack of apoptosis propensity.
Thus, there is strong evidence for the involvement of the innate immune system and at least one other pathway in the pathogenesis of AMD.
We also failed to find any role for the two other main pathways of the RNAi machinery, or for Toll and Jak-Stat signaling.
