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Cells were maintained as monolayers in 75 cm flasks and passaged two or three times weekly by scraping and diluting the cells into fresh DMEM with 5% foetal bovine serum.
Two or three bites.
Or two, or six.
Two or three grand?
Two or three dozen?
Choose two or three.
two or three towels.
If the stain is not completely gone, repeat steps 2 through 4. Hang one or two teabags in the flask.
Eight T25 flasks (one for each time point of 2, 6, 12, 18, 24, 36, and 48 h and mock-infected) per strain were inoculated and placed at 37°C in 5% CO2 [17].
For control on expression of CD73, CD90, and CD105, human synovium-derived cells from RA patients were cultured in plastic culture flasks for three or five passages.
Each clone was split into two parallel flasks, in one of them cells were grown without doxycycline.
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