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We have also tested two mutants of OBP14.
We here compared the binding parameters of two mutants of the melanoma antigen peptide MART-127 − 35 specific TcR DMF5.
A total of 10 single-site mutants were chosen using the PoPMuSiC program, and two mutants of K253N and P314T showed enhanced thermal stability.
The inhibitory effect was more pronounced on two mutants of PfTIM (Y74C and Y74G), both of which have reduced stability compared to the wild-type protein due to an interface cavity.
We designed two mutants of superoxide dismutase (SOD), one is thermostable and the other is thermolabile, which provide valuable insight to identify amino acid residues essential for the thermostability of the SOD from Aquifex pyrophilus (ApSOD).
In this study, two mutants of S. Choleraesuis were constructed and their attenuation and intracellular fate analysed with the purpose of engineering new attenuated live strains with improved properties as oral vaccine carriers.
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Four mutants of S. Typhimurium, with Tet-conditional growth, were created by inserting the tetRA cassette.
The six mutants of pepsin were subsequently constructed and examined for their catalytic activities.
Seven independent ERA mutants were recovered using Mabs directed against antigenic sites I (four mutants) and IIIa (three mutants) of the glycoprotein.
In total, nine mutants of FP2A were constructed to test the proposed importance of seven different amino acid residues.
In our previous studies, we isolated almost twenty mutants of kayvirus 812, which, when used in cocktails, extended the amount of susceptible S. aureus strains of different origin1,27,28.
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