The heterokaryotic nuclear fusion (0.11%) of two MCF-7 subtypes was identified and quantified by flow cytometry.
The potential of STX243 to inhibit the growth of paclitaxel-resistant tumours was investigated using two MCF-7 (ER+) cell lines.
Two MCF-7 cell lines were established with acquired resistance to CJM 126 (IC50 values > 20 microM), which exhibit cross-resistance to substituted benzothiazoles, but equal sensitivity to tamoxifen and doxorubicin.
To calculate differential expression, edgeR requires only a pair of replicates in one condition, allowing its use with the single MCF-10a sample and two MCF-7 replicates of this study.
Two MCF-7 stable clones expressing PI3K p110αCAAX were generated in the laboratory of Dr. James McCubrey at East Carolina University [ 13] and maintained in 89% RPMI (Invitrogen, Carlsbad, CA), 10% FBS, and 1% penicillin/streptomycin.
Indeed, where replicate data is available, this information is useful and can lead to improved performance, as evidenced by the closer correspondence achieved between the RNA-Seq and the array data with edgeR and utilising the two MCF-7 replicates.
To assess whether overexpression of GABARAPL1 regulated the growth rate of breast cancer cells, we performed a kinetic viability assay (MTT) using wild-type MCF-7, MCF-7-pcDNA3.1 and two MCF-7-Flag-GEC1(His 6-expressing clones.
Our proteomic analysis of two MCF-7 human breast cancer cell lines revealed at least seven differentially expressed protein spots in two-dimensional gels, which may contribute to the mechanisms of MEK5/Erk5-mediated TNF-α resistance.
Two MCF-7 transfectants (transfected either with pcDNA3-EGFP/YB-1 or with pDsRed2-N1/YB-1, Figure 2) were co-cultured (1 to 1 ratio) with or without 10 nM doxorubicin for 6 days and visualized by phase contrast and fluorescence microscopy.
Arabidopsis thaliana encodes three MCF proteins (AtAPC1-3) that represent promising candidates for net adenine nucleotide transport.
