Elsewhere on shelves were a leg of lamb; a rabbit carcass under a layer of conifer sprigs; a single cooked lobster on a bed of ice; swordfish ham; a few pieces of salmon, air-sealed in sycamore sap; a pork shoulder brining in pine-needle juice; four marrow bones in a bag with mustard greens.
Constitutional/control DNA consisted of buccal mucosa and lymphoid fractions of the patients and one marrow sample without evidence of MDS sorted into blastic, erythroid, and myeloid fractions (see Table S1. in supplementary material).
Twenty-three patientsubsequentlyly received a transplant (eight allografts, eight marrow autografts and seven peripheral blood stem cell transplants).
This method requires only 2 h for the analysis of three million marrow cells from the autograft, and is more effective than alkaline phosphatase staining with the same monoclonal antibodies.
Lymph nodes were a first site of progression in 10 patients, breast in nine, CNS in seven, intestine in three, lung in three, bone marrow in two, and liver, nasal cavity, ovary, adrenal gland each in one patient.
Two million bone marrow cells were then differentiated into macrophages using 20 ml complete medium supplemented with murine 40 ng/ml rM-CSF (PeproTech).
Results: Ten clinical samples (4.3%) gave invalid LC results, including three of five bone marrow samples but only two of 165 serum samples.
This side effect, called graft-versus-host disease, kills one marrow-transplant patient in three.
More than 10 million synovial MSCs were obtained from all nine donors, contrary to more than 1 million bone marrow MSCs from only two among nine donors [ 19].
In patients with secondary MDS, nine received marrow and eight received G-CSF mobilized PBSC as a source of stem cells.
