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Two linkage agents were used: glutaraldehyde and glyoxal.
The legs are composed of a supporting columnar linkage and two linkage connecting it with the body as shown in Fig. 13b.
The third formulation is another combinatorial approach involving the above two linkage decompositions by Wood (2009), which we term 'method 3'method
Consistent with the segregation ratio, we identified two linkage regions on chromosome 7 and 8, respectively (Additional file 1: Figure S2B).
The legs are composed of the supporting columnar linkage and two linkage connecting it with the body, as shown in (b).
Using the MutMap gene mapping strategy, we identified two linkage regions on chromosome 7 and 8, respectively, consistent with the segregation ratios in the F2 population.
A linkage configuration, joining two carriages by two linkage bars, is configured to form a symmetric inverted crossover four-bar linkage.
A comparison of the QTL mapping efficiency between the two linkage maps revealed that the high-density bin map had higher resolution.
Pair-wise linkage disequilibrium was evaluated by two linkage disequilibrium parameters, Lewontin's D2 [24] and r2 [25], which were both calculated in haploview (http://www.broad.mit.edu/mpg/haploview/) [26].
This results in two linkage maps per chromosome that are then juxtaposed and integrated based on common MI and codominant markers and their average distances.
Two linkage files were used.
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