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If an ISC hypocentre was produced, then it has always been reviewed by at least two ISC analysts.
Two ISC experiments with fractures oriented perpendicular and parallel to the air injection direction were conducted at identical initial and experimental conditions.
How asymmetric N signaling between two ISC daughter cells is established has remained poorly understood.
Fate determination between the two ISC daughter cells is regulated by N signaling (Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2006, 2007; Bardin et al., 2010).
As long as two ISC daughter cells transduce different levels of BMP signal, the N and BMP signaling feedback loops can amplify the initial small difference, leading to a bistable cell fate choice.
DOI: http://dx.doi.org/10.7554/eLife.01857.006 To determine whether BMP signaling controls ISC/EB fate choice more definitively, we carried out lineage tracing experiments in which the two ISC daughter cells and their descendants were labeled by RFP+ (red) and GFP+ (green), respectively, following FRT-mediated mitotic recombination.
Similar(52)
There is evidence of two different ISC populations (Barker et al., 2007; Sangiorgi and Capecchi, 2008).
The two models (ISC vs. CAS) produced comparable IBS-like symptoms and changes in the BGA in rats.
The subsequent assessments of the two typical ISC metabolic enzymes were performed according to the previously reported protocol.
For IFA, tissue sections were deparaffinized and blocked with 5% goat serum in PBS with 0.05% Tween 20 (ISC Biosciences) (blocking buffer) for 1 hour at 37°C.
The activity of two typical ISC-dependent metabolic enzymes, aconitase and complex I, in cells transfected with miR-210 mimic and inhibitor was measured.
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