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Equal amounts of RNA from two individual fish livers from the same tank were pooled together and analyzed as a single biological replicate, providing two replicates per tank and six replicates per dietary treatment.
Similar(59)
A recent survey at that site found eight individual fish at that site, prompting scientists to estimate the reef housed 20 to 40 handfish.
For six individual fish covering a range of body sizes (210 368 mm TL), digital photographs of the dissected jaw anatomy were taken from the fish's right side with a scale bar in frame.
Twenty barramundi fish averaging 6.7 ± 0.1 g (mean ± standard deviation) were randomly distributed in fifteen individual fish groups to each of fifteen aquaria.
As many as fifty individual fish may gather at a single, plankton-rich feeding site.
Even within a school of fish caught in the same location, the degree of contamination can vary greatly from one individual fish to another.
For each sampling, 6 replicates of fish were used, each replicate consisting of four individual fish (24 fish per group).
Typically one pooled sample was prepared from three to five individual fish.
Cells cultures were generated from six individual fish, this allowed purification of sufficient cells for two six well plates.
For each studied tissue, cDNA originating from three individual fish were pooled and subsequently used for real-time PCR.
For each treatment, eight individual fish were exposed for 96 hr on a 24-hr static renewal schedule.
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