Always leave a minimum of two hour separation between adding different chemicals to the tub to prevent any negative chemical reaction and to maximize the effect of your chemicals.
Establishing radio contact two hours after separation is another of those high anxiety moments.
"So, the reception of the signal by Rosetta from the lander roughly two hours after separation is for me one of the most critical moments, also".
The first images from the instrumented Impactor were seen two hours after separation.
After induction of anesthesia before surgery, and two hours after separation from CPB we collected the bronchiolar lavage (BAL) fluid.
Peripheral blood cells and alveolar lavage fluid were collected directly after induction of anesthesia and two hours after separation from cardiopulmonary bypass (CPB).
Arterial blood acetate, gluconate and interleukin-6 (IL-6) levels were measured immediately before CPB (T1), three minutes after CPB commencement (T2), immediately before CPB separation (T3), and four hours post separation (T4).
Arterial blood was sampled at four time points: T1, immediately before commencing CPB; T2, two minutes after commencement of CPB, prior to placement of the aortic cross-clamp; T3, on rewarming, just prior to separation from CPB; T4, four hours after separation from CPB. Measurements at each time point included plasma acetate, gluconate and IL-6 concentrations.
Expression of hfq was quantified (using primers Q_Fn_hfq-84F/Q_Fn_hfq-195R) fRNA RNA extracted at 20 minutes, one hour, and four hours post-separation and compared to hfq levels from time 0 (pre-separation).
If you remember, this radio hook-up was about two hours after the pair's separation, but still five hours from Philae's eventual touchdown.
The separation procedure required two hours, and the cells were used immediately after isolation for the experiments described.
