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The Cx. quinquefasciatus colony recovered rapidly after tetracycline treatment and was easily expanded to normal numbers of animals within two generations without any obvious reductions in fertility, fecundity, or viability.
After having established the linkages, the genetic gain can be accelerated by scoring for markers associated with the QTLs for two generations without phenotypic observation.
The authors also showed that H3K9me3 at the target locus persisted for two generations without any additional input of dsRNA, while the level of small RNAs rapidly dropped after the first generation, consistent with small RNAs bringing about histone modifications.
Uncontrolled environmental effects during the subsequent holding phase might affect life histories, so the comparison of different traits among the selection lines was performed only after keeping the selected fish for at least two generations without any further selection under controlled and time-matched conditions.
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We make glancing acquaintance with members of three generations without gaining real intimacy with any of them.
This resistance was unstable after five generations without selection.
The founder was crossed to wild-type individuals, and the mutant line has been maintained for over five generations without any changes in phenotype.
These embryos completed their development and could be propagated perpetually in 90% O2, demonstrating that hyperoxia tolerance was maintained for at least five generations without further selection.
hESCs were passaged in feeder-free medium supplemented with cocktail inhibitors for at least three generations without obvious differentiation (Figure 5F) and restored differentiation into three cell lineages following drug removal (Figure 5G), suggesting reversible effect of the inhibitors.
As the sampling procedure stringently followed the inclusion and exclusion criteria that emphasized the three generations without any different ethnic admixture rule for an individual to be considered as a valid subject for this study, we assumed that there was no recent admixture or gene flow among all the studied populations.
The AnTc-grown population was then diluted 10-fold and grown for three to four generations without AnTc.
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