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A calculated amount of the second seed culture was centrifuged at 4 °C and 5000g for 10 min to harvest the cells, which were washed in the YNB medium with two further cycles of centrifugation (4 °C, 5000g).
Stromal layers were treated with two further cycles of pipetting.
If in a progressive PR at six cycles, two further cycles of CHOP were given.
At 1 month after the completion of radiotherapy, two further cycles of ECF chemotherapy were given.
Patients who gained a complete remission (CR) or good partial remission (GPR) were then randomised between two further cycles of PVACEBOP chemotherapy, or an autologous haemopoietic stem cell transplant (HSCT) with etoposide and melphalan preconditioning.
At aged 45, after another period of amenorrhoea she had a long, ovulatory but deficient, cycle of 63 days followed by two further cycles of normal length but deficient luteal phases.
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Resected patients received six further cycles of chemotherapy postoperatively.
Eighty-nine patients underwent treatment stratification, 45 were randomised to receive twelve further cycles of weekly docetaxel and 44 to receive four further cycles of 3-weekly docetaxel.
Two patients with stable disease after six cycles of the combination received six further cycles of temozolomide alone.
This patient experienced multiple grade 1 AEs in the first cycle, but later received three further cycles at full dose.
Those patients who underwent resection of their liver metastases received six further cycles of the same chemotherapy regimen postoperatively.
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