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Adsorbed proteins were then eluted by gradient elution with ammonium sulfate (from 30, 20, 10, 0% of saturation) at a flow rate of 2 ml/min, and each gradient was eluted with two fold column volume of buffer.
Unequal sample size for each gestational week did not discredit our data, because at least 20 pregnant women for each gestational age was adequately calculated and our enrolled population were more than two fold of this volume.
Chest CT showed that all patients had a two fold increase in lesion volume within the first three days despite broad spectrum antibiotic treatment.
The marking intensity increased considerably when nymphs were forced to feed the same volume of two fold concentrated sera (Figure 1-E).
The problem is two fold.
It's two fold.
In addition to automation, the developed sequential injection analysis method greatly improves the standard off-line oxalate complexation and alkalimetric titration method by reducing thousand fold the required sample volume, forty times the nuclear waste per analysis as well as the analysis time by eight fold.
γ-T3 reduced the tumor volumes in a manner approximately two folds and the effect is similar to PSP.
The viable count was determined by counting the number of colonies formed on LB agar medium inoculated with 10 μL volumes of ten fold dilutions and incubated at 37°C for 24 h.
About 24 h after venipuncture, plasma and buffy coat of the blood samples were removed by centrifugation (1,500 g) and aspiration followed by washing three times in a 10-fold volume of RPMI 1640 medium (1,500 g, 3 min, 4°C).
Erythrocytes were washed three times in a 5-fold volume of cold saline (pH = 7.4) with subsequent centrifugation at 4°C for 5 minutes at 2862 g.
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