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In the COX++ versus Col-3 comparison, the number of differentially expressed genes was 485 (2.1% of total) of which 13 (0.1%) were more than two fold up- and 85 (0.4%) were more than two fold down-regulated.
Only 131 genes (0.6% of total) were differentially expressed in both modifications of which 47 (0.2%) were more than two fold up- or down-regulated in at least one of the modifications.
Differentially expressed genes were selected by comparing the expression profiles of the six horses, with a selection criterion of more than two fold up- or downregulation in four or more horses.
An empirically setting of statistical thresholds for fold changes between samples, which is most widely used in published studies (we assumed more than two fold up- or downregulation as significant).
Comparing COX + lines with Col-3 plants, 545 probe sets (genes) were differentially expressed (2.4% of the total) of which 139 (0.6%) and 35 (0.2%) were more than two fold up- or down-regulated in COX + lines, respectively.
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In the SA signalling pathway, transcripts of a phenlyalanine ammonia lyase (PAL) homolog were two fold up-regulated, whereas isochorismate synthase (ICS) did not change significantly.
Fifteen CYPs showed more than two fold up-regulation, while 24 showed down-regulation at 15°C as compared to at 25°C (Additional file 14).
Pregnancy specific β1 glycoprotein 9 (Pshowedhowed a consistent two fold up-regulation in adenomas compared to normal mucosa and as the most statistically significant candidate in these experiments (p < 0.006) was selected for further analysis (Fig. 1b).
Of 31099 genes on the microarray, 6908 transcripts were expressed at a level above the threshold defining differential expression (see Methods) and at least two fold up-regulated over the other groups.
Barbara Hays, a retired advertising executive who travels frequently from the Midwest to Montana, said she relied on her two fold-up Longchamp totebags — a large one that measures 12 by 12 by 7 inches and costs $145, and a smaller one that measures 10 by 10 by 5› inches for $125.
Outside of her office, she has two fold-up tables that covered in pieces of plastic, scraps of paper and textiles, plaster, and more "stuff," as she calls it; her sculptures are made from just about anything she can find.
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