Sentence examples for two fold dilution from inspiring English sources

For the first 10 passages the cells seeded in coated wells of either equivalent or double surface in order to achieve a two fold dilution.

Two fold dilution series of the cDNA template were assayed to generate standard and disassociation curves.

Striatal B with different concentrations (0.04 to 20 μg mL−1, two fold dilution) was coated to the microtiter plate and then bound to pAbs with serial dilutions (1 50 to 1 6,400, stepwise in two fold dilution).

A series of two fold dilution of each oil, ranging from 0.2 to 25.6 mg/ml, was prepared in Muellur Hinton agar at 48°C.

Working solutions were made up by two fold dilution of the stock and 20 μL of each concentration was added to each well in triplicates.

Reported concentrations of MMP-12 were not adjusted for this two fold dilution with Buffer A. Plate washing was performed with a Biotek (Winooski, VT) ELx405 96 channel plate washer.

To determine the 50%% inhibitory concentrations (IC50) of these primary hits on Wnt signaling pathway, we performed secondary screening using three concentrations by two-fold dilution, with the highest concentration being 20 μM.

Melittin was tested at eight concentrations, with two-fold dilution from a concentration of 20 μM.

Efficiency was determined for each primer pair using a two-fold dilution series across five points for five patient samples of varying RNA integrity.

Compounds were then serially diluted by two-fold dilution from well B3 till B11 by transferring 50 μL to the next well after proper mixing.

The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EEO against the four pathogens was determined by the two-fold dilution method described by Kubo et al. ([2004]), with slight modification.