Of the five clodrolip treated tumors 2 were identified as non-responders, whereas the remaining three displayed 2-6 foldepletionof of macrophages relative to their size matched control tumors.
Further support comes from B52 depletion experiments showing a two fold reduction of ey(2a) and concomitant increase of ey mRNA levels.
A three fold increase in telomere-DSB fusion frequency with γ-rays (p=0.023) and a two fold increase with 1 GeV Fe ions (p=0.14) was observed with tankyrase 1 depletion, increases similar to those seen with DNA-PKcs inhibition or the two treatments combined (all p < 0.023).
Fold depletion of the siRNA-target transcript over controls (ACTB, GAPDH) was measured by qPCR.
Fold depletion of the target transcript over controls (ACTB, GAPDH) was measured by qPCR.
The answer is mainly two fold.
Such genomic regions were further filtered to include sequences with minimum 1.3 fold enrichment, and minimum −1.3 fold depletion.
Our rationale for this is two fold.
two fold above background.
