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Adsorbed proteins were then eluted by gradient elution with ammonium sulfate (from 30, 20, 10, 0% of saturation) at a flow rate of 2 ml/min, and each gradient was eluted with two fold column volume of buffer.
Similar(59)
It's two fold.
The answer is mainly two fold.
The problem is two-fold.
The fold column was generated from mFold [ 30].
In theory, the method used for screening library #1 only required an average of 120 reactions: one 8-fold column pool, plus eight individuals for each of three mutations detected (24 additional reactions).
The first two folded.
One fold, two folds, then a third.
Column fractions are concentrated ten fold by TCA precipitation, and analyzed by SDS-PAGE followed by Western blotting with anti-Unc45b and anti-Hsp90β antibodies.
In average, a three fold reduction in the liquid backmixing was achieved in the trayed column as compared to the column without the trays.
At high temperatures vapour from the liquid phases was shown to produce up to a four fold increase in local gas holdup on moving up the column.
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