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One to two extra hormonal stimulation cycles were initiated to obtain an accumulated minimum of 6 oocytes (group A) or alternatively of 5 embryos (group B) for each patient.
The PCR cycle conditions were as follows: one cycle of 95°C for 5 min, followed by 35 cycles of 95°C for 30 sec, annealing temperature for 30 sec, 72°C for 45 sec; and finally one extension extra cycle of 72°C for 10 min. For the screening analysis, amplicons were visualized in agarose gels stained with ethidium bromide.
Protecting left-turn movements on all four approaches to a signalized intersection conventionally requires a minimum of two extra phases per cycle.
Republicans fell short in their quest to take control of the Senate, with Democrats picking up two extra seats in this election cycle.
Surprisingly, we found that ∼3% of centrioles proceeded through an extra round of template-driven replication within a single nuclear cycle, and a few of these centrioles proceeded through two extra rounds of replication.
Maybe one or two extra.
"It only takes up two extra characters".
Two extra user features were evaluated.
We prepared two extra control samples.
Two extra words.
This resulted in two extra runs.
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