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We also performed reporter gene assays where CAT expression was driven by two DRE sequences.
The second largest group consisted of AP2-domain transcription factors (8), including two DRE binding proteins DREB1B (CBF1, At4g25490) and DREB2A (Athat5410) thavehave been well characterized in regulation of cold responsive and dehydration responsive genes, respectively [ 7, 41].
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Yeast one-hybrid assays and invitro electrophoretic mobility shift assays further showed that DREB2C interacts with two DREs located in the HsfA3 promoter with a binding preference for the distal DRE2.
At least one DRE system has been shown to be vulnerable to infection using the activation card.
In a normal cell cycle, RNR3 expression is inhibited by the transcriptional repressor Crt1/ Rfx1, which binds to three DREs located upstream RNR3 ′ s promoter (Huang et al. 1998).
The 1.74 kb sequence 17-1 [GEU161097]U161097] contains four DRE-like, one ICEr1-like, and four ABRE elements, which is significantly more than any of the other 5'-tagged regions.
Briefly, the regulatory sensor sequence consists of two parts: a) a 484-bp fragment from the upstream region of the mouse Cyp1a1 gene that contains four DREs, and b) a 1,330-bp 1,330-bp fragmentt ofrome mouse mammary tumor virus partofer lacking glucocortheoid-responsive enhancers.
They have identified four cis-regulatory elements, DRE, DRE-core, and ABRE for the drought response, in addition to AS1 (not shown) that is a functional element not involved in the drought response.
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To verify the expression profiles of known miRNAs, we selected four miRNAs (dre-miR-456, dre-miR-22a, dre-miR-206, and dre-miR-192) from the last group based on their known or potential roles in zebrafish early development.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com