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The Pain-1 sequences obtained from the same BAC by two different sequencing techniques (454 and Sanger sequencing) differed by a six-nucleotide insertion in intron 2. The Pain-1 gene consists of seven exons and six introns and is around 4 kbp long.
The ability of the platform to detect these mutations in 50%, 25%, 10%, and 5% dilutions of DLD1 DNA was assessed in two different sequencing runs performed on different Ion Proton sequencers.
In Table 2 we evaluated the performance or running times of these algorithms on four real-world datasets from two different sequencing platforms: 10.9 million 36 bp reads from an Illumina 1G sequencer, 3.5 million 55 bp reads from an Illumina GAII sequence, 1 million 25 bp reads from an ABI SOLiD Sequencer, and 1 million 50 bp reads from an ABI SOLiD sequencer.
To increase the rigor and general applicability of these studies, cohorts A, B and C were sequenced in two different sequencing facilities.
We used two different sequencing facilities and independent patient cohorts in order to address generalizability and reproducibility issues, both critical for biomarker development.
Out of the six Musaceae species evaluated using the two different sequencing approaches, only M. textilis gave polymorphic ITS sequence after direct Sanger sequencing.
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The electrocoagulation (EC) reactor was tested in two different sequences (before and after the flocculation unit).
The influence of components separation was treated with two different simulators using two different sequences of distillation columns.
The two different sequences of events leading to biparticle chaining of equal-sized and different-sized latex microspheres are sketched in Fig. 1b and 1c.
No statistical differences in changes in pain and symptom intensity during switching and between the two different sequences were observed.
This result indicates that the mild group showed one to two different sequences in five trials.
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