Sentence examples for two different polymerase from inspiring English sources

Nasopharyngeal aspirate was obtained and processed for viral antigens using the direct fluorescence antibody (DFA) assay, and two different polymerase chain reaction (PCR) viral tests.

The samples were assayed for HHV-DNA (HHV1 8) by using two different polymerase chain reaction (PCR) assays, qualitative PCR (multiplex PCR) and quantitative PCR (real-time PCR).

Therefore, to obtain some baseline data for Poland, the prevalence of C. psittaci in various parrot species that did not exhibit clinical signs of infection was determined using two different polymerase chain reaction (PCR) amplification assays.

In the Gram-positive bacterium Bacillus subtilis and related micro-organisms, this machine contains two different polymerases which are thought to be specialized in leading (PolC) and lagging (DnaE) strand polymerization [4], [5].

Moreover, full length primer extension experiments were performed to investigate the similar sequence effects on in vitro translesion synthesis by two different polymerases: high fidelity replicative polymerase exofree Klenow fragment (Kf-exo–) and error prone specialized bypass polymerase Dpo4.

Assuming that also two different polymerases are used in human, we suggest that the observed asymmetries are due to a difference between the error spectra of these two DNA polymerases.

Alternatively, the difference between the mutational spectra of the two DNA strands can be a result of replication of the two DNA strands by two different polymerases (Kunkel and Burgers 2008) that have different error rates for insertion of nucleotides in the nascent strands (Kunkel and Bebenek 2000; McCulloch and Kunkel 2008).

Fig. 3 PCR of comp15071_c0_seq5 (Lcc5B) with three different polymerases.

We have prepared another manuscript on the evolutionary scenarios of these four different polymerases, as well as analysis of their relationship in a context of both bacterial taxonomy and sequence evolution.

Moreover, three different polymerases were used, namely GO Taq DNA Polymerase (Promega, Madison, WI, USA), DNA Taq Polymerase (Sigma-Aldrich St . Louis USA), and recombinant Taq Polymerase (Fermentas), to reveal that only the latter one was suitable enough for the designed PCR reaction, whereas the use of two others resulted in PCR reactions giving non-specific bands of different sizes.

Two different quantitative polymerase chain reaction (Q-PCR) experiments were conducted with UFPs and water-soluble and-insoluble fractions.