Epitope-tagged TP03_0882 expressed in mammalian cells was found to translocate into the nucleus, a process that could be attributed to two different nuclear localisation signals.
Furthermore, there are several similarities in the miRNA expressions of SCC in spite of two different anatomical localisations.
Next, we analysed the localisation of two different histone modifications; H3 lysine 4 dimethylation (H3K4me2), known to be associated with active or potentiated chromatin, and H3 lysine 9 trimethylation (H3K9me3) as a marker of inactive chromatin.
Two different computational analyses suggested a putative localisation to the membrane of survivin-ΔEx3, with inside-out orientation of the C-terminus domain.
From the images shown in Figure 1A it is obvious that the three U1 snRNP-specific proteins show different localisation patterns in the nucleus.
Nineteen samples of oral leukoplakia, six samples of lymph node metastases, and one hundred and two samples of head and neck carcinomas of different localisation were collected.
The EGFR protein expression levels and subcellular localisations were examined by two different anti-EGFR antibodies: clone 5B7 against the intracellular domain and clone 3C6 against the extracellular domain, hereafter referred to as intracellular and extracellular domain antibodies, respectively.
We have found similar dynamics in all three conditions, despite the different tags and their different localisation within the protein.
Localisation of telomerase was examined in two different ways: firstly, by in situ TRAP assay, using fluorescent primer as its substrate and, secondly, by double staining of telomerase and nucleolin, a protein that is typical of nucleoli.
Furthermore, RIFIN variants have different developmental expression in merozoites where members of the two sub-families remain within the parasite cell although exhibiting different localisation patterns [ 17].
