By plotting the difference between the pEC50 values determined by each of the methods against the mean of the two determinations it is possible to determine the agreement between both methods [ 10].
The different results reported in the paper are the average of minimum of two determinations.
Two determinations are needed for something to be spatially real: being in space and being extended in space.
These two determinations, however, lead to a contradiction involving external negation (See Routley 1980; Simons 1992, 185; Thrush 2001b).
The data presented in top panel were from one of two similar experiments, and the data in bottom panel are means of two determinations.
All psycho-static investigations are ultimately based on two determinations of psychic magnitude: (1) the "inhibition-sum" (Summe der Hemmung or Hemmungssumme); (2) the "inhibition relation", or better, "inhibition-ratio" (Hemmungs-Verhältniß).
For the irregularity indexes, random errors were calculated according to Dahlberg's formula (Se2 = Σd2/2n), where Se2 is the error variance and d is the difference between two determinations of the same variable [33].
The random errors were calculated according to Dahlberg's formula (Se2 = Σd2/2n) [27], where Se2 is the error variance and d is the difference between two determinations of the same variable and the systematic errors with dependent t tests, at P < 0.05 [28 30].
Systematic errors were evaluated with dependent t tests at p < 0.05, and casual errors were calculated according to Dahlberg's formula (Se2 = Σd2/2n), where Se2 is the error variance and d is the difference between two determinations of the same variable [25].
In addition, it is better to correlate the two determinations using the same fusion protein.
All the samples were tested in duplicates, and results were expressed as the mean values for the two determinations.
