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We compared two depletion techniques.
The ligand dependence is revealed when there exist two depletion pathway of the activity, one of which exhibits a non-first order property (Fig. 10).
We named and ranked proteins following two depletion strategies.
To demonstrate InteQuan's ability to handle the variation in the total protein concentration, six samples were diluted into three concentrations (equivalent to the loading of 15, 20, or 30 μl of the original plasma samples: see Methods) and analyzed in duplicate using two depletion columns along with eight aliquots of the HPS sample in Study III.
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Notably, (bar{lambda }_{text{m}}) and (bar{beta }_{text{m}}), two depletion-driven parameters, are simply dependent on average pressure and bottom-hole pressure and do not require pseudo-time, making this method easier to implement than the pseudo-functions-based approach.
In the first two depletions the increase in Golgi number comes from the splitting of Golgi stack pairs [8], whereas upon SMSr depletion this is accompanied by a Golgi disorganisation into tubular-vesicular clusters [9].
Freshwater storage depletion in the brackish aquifer was expected to reduce the supply by 10% with 99.5% reliability compared with zero depletion.
By day five, depletion of α-SNAP caused cell rounding in adherent cell lines; we therefore restricted our analysis to adherent cells at early time-points.
To determine whether InteQuan can better control analytical variability during use of multiple depletion columns on clinical samples, 18 clinical samples in triplicate along with 12 aliquots of the HPS sample were analyzed in three experimental batches using three depletion columns in Study II.
Two different depletion strategies for removing albumin from human cerebrospinal fluid (CSF), Microcon Centrifugal Filter vs. Montage Albumin Deplete kit, were evaluated for improving protein profiling pattern and reproducibility in SELDI analysis.
The tumors evaluated in this study were selected from two independent depletion experiments.
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