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Studio musicians complemented the accompaniment from the January 6 session with two trumpets, two oboes, two cor anglais (English horns), and a double-bass.
In parallel, soluble sugars and proline contents were determined along with expression profiles of two COR genes in plants exposed to low temperatures.
In addition, the cellular concentration of soluble sugars and proline were determined, along with the transcript accumulation profiles of orthologues of the major vernalization regulator VRN1 and two COR genes at different stages of cold acclimation.
These include two COR (COld Regulated -related genes, NCED3 involved in ABA biosynthesis, the AFP1 gene encoding an ABI5 binding pRegulated -relatedhock transcription factor HSFA2, a PIRIN genesiNCED3ed involvedinnABAng, biosynthesisved in regulatheg proline dehydrogenAFP1 and ECA2 encodingene encodingtransporter involved in stress responses.
Of note, IL-10 levels were all the same for the Mal d 1 proteins, but the two Cor a 1 derivatives FV and CFV completely failed to induce IL-10-producing cells (see Fig. S4 of the article's online Supporting Information).
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In order to investigate whether the polymorphism in the coding sequences of the CBF genes has a general effect on the expression of the target COR genes, five COR genes (COR6.6, COR15a, COR15b, COR47 and COR78) were chosen for analysis (Fig. 5).
All five COR genes were expressed in all accessions, but again, sensitive accessions generally had lower COR gene expressions levels, except for Ita-0 (157 AV) (Fig. 5).
In this study, CdICE1 overexpression resulted in elevated expression of CBF and three COR genes during 4°C cold acclimation and enhanced tolerance to freezing stress.
The five COR genes contain the consensus DRE element, to which the CBF proteins have been shown to bind and are therefore direct targets of the CBF proteins.
Expression of the three COR genes was also significantly higher in CdICE1-overexpressing lines compared with WT plants undergoing acclimation at 16°C or 4°C.
HIV infection was significantly more prevalent in those women whose husbands had attended primary school (crude odds ratio [COR] [95% CI] =2.71 [1.18, 6.25]) and had a family size of three to four members (COR [95% CI] =1.85 [1.02, 3.90]).
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