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The other three blacks include a sports writer and two copy editors.
ddPCR results were affected by the use of single vs. two copy reference genes.
I hired two copy editors to go through the basics on spelling and grammar.
The expected ddPCR values were underestimated when CruA and Ccf (two copy endogenous Cruciferin sequences) were used because of high number of copies.
On the other hand, real-time quantitative PCR results were not affected by the use of single vs. two copy reference genes.
It is important to make an adjustment if two copy reference genes are used for ddPCR in order to obtain accurate results.
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For the sake of simplicity, we can assume to deal with two copy-moved patches without loss of generality.
For the sake of simplicity, we will assume, without loss of generality, to deal with two copy-moved patches (i.e., no multiple copies).
Several oligonucleotides have an intermediate value between one and two copy losses (Figure 2, Figure S1, Figure S2, Figure S3, Figure S4, Figure S5, Figure S6, Figure S9).
There were 3.4 times as many two-copy retention genes than that of three copy retention genes.
On each plate two calibrator samples were included; a two-copy sample from a healthy control and a one-copy sample from a penis cancer case.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com