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A compound was considered a secondary hit if it produced a significant increase in lifespan at two consecutive concentrations.
A previous publication of the "Gesellschaft für Umwelt-Mutationsforschung" defined a dose effect relationship as follows: if there were at least two consecutive concentrations having increased aberration frequencies and the effect of the higher concentration was more pronounced than that of the lower, this was regarded as dose effect relationship.
To capture activity when a dose response curve was nonmonotonic or could not be calculated from these criteria, a special case AC50 was assigned to the first concentration when a chemical inhibited an assay by at least 50% at any two consecutive concentrations.
Similar(57)
Two consecutive concentration-response curves to acetylcholine (3 nmol/l–10 µmol/l) were studied in control and in the presence (30-min incubation) of one of the following inhibitors: losartan (3 µmol/l), apocynin (100 µmol/l), tiron (1 mmol/l) plus diethyldithiocarbamate acid (DETCA, 100 µmol/l), and captopril (100 µmol/l).
For example, a bee extending its proboscis from 0.3 to 30% has an aversive score of 5 as it responds to five consecutive concentrations.
The estimation was independently computed only on short intervals of three consecutive concentrations of the calibration curve.
Therefore, for the estimation of the short calibration interval (three consecutive concentrations), the linear approximation is sufficient.
For small intervals of three consecutive concentrations, it is sufficient to use approximation via a linear function.
As far as the concentration of A10-2 in our experiment was concerned, the binding capacity of A10-2 with GP73 changed most obviously in three consecutive concentrations of GP73 (2, 1, and 0.5 μg/mL).
The interval of the three consecutive concentrations was selected at low concentration values for the three ions, that is, the protonated MCYST-LR molecular ion, doubly charged sodium adduct of the MCYST-LR molecular ion, and the molecular ion with cleavage of the Adda moiety.
Once the concentration became stable, defined as two consecutive trough concentrations within the target range, trough concentrations were measured every 4 6 weeks.
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CEO of Professional Science Editing for Scientists @ prosciediting.com