For 3%H2O22 treatments, no significant difference was observed between the two LPS concentrations, which may be because H2O2 induced cell death became predominant.
Intra-day precision and accuracy was determined by assaying standard solutions of eight boswellic acids at three different concentrations which fall within the range of the calibration curve.
Data in Figure 1 provide examples from three CER and SM concentrations, which mirror the trend found for the lipids measured (Table 1).
Miniaturization of this assay in 1536-well plate format enabled an initial quantitative high-throughput screen (qHTS) of over 70,000 small molecules tested at seven different concentrations, which identified structurally unique compounds showing anticancer activity, including the 8-hydroxyquinoline chemotype subsequently also identified and developed by others.
In addition in between the α- and the β-relaxation a β′-process is observed for the polymers with the two lowest azobenzene concentrations, which seems to be related to the azobenzene unit.
Three samples of known concentrations which span the assay range were assessed 20 times as a measure of the intra-assay precision.
For all further analyses of the two different phenotypes, two concentrations were employed which induce either the aneuploid phenotype, i.e. 2.5 nM SAG or 4 nM PAC or the mitotic arrest phenotype, i.e. 40 nM SAG or PAC.
Following incubation and two PBS and water washes bound salivary proteins were eluted with two concentrations of SDS, which were later analysed using SDS-PAGE and Western blotting.
Fifty eight parameters have been identified, together with five initial conditions of metabolites concentrations which were not known from experimental data (pgp = 1.215 × 10−5, 3pg = 1.911, xyl5p = 1.564, sed7p = 4.085 × 10−2, and g1p = 6.500 × 10−2).
The six concentrations at which samples were spiked were unique for each cytokine with the lowest spike ranging from 2 43 pg/ml and the highest from 1191 8062 pg/ml).
