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A cage is manoeuvred into position and two bait balls go out, attached to ropes: one on the surface and one a few metres below.
The authors recommend a minimum of two bait stations per urchin trap to optimize CPUE.
Two trials were conducted to test the following: i) the efficacy of three sea urchin trap types ii) the effect of trap soak time (1-8 dand) and iii) the efficacy of two bait types fed at various quantities.
Additionally, TPP1 and TIN2 were repeatedly found in the POT1 datasets (4 of 5) with significantly more spectra obtained compared to the other two bait proteins (Table S1); another general indicator of enrichment as the more protein there is in a sample, the more MS/MS spectra obtained.
[24] HEK293T cells expressing receptor DHFR chimera and FKBP12 prey were treated with the cytokine ligand and a concentration gradient of either of the two bait MFCs.
Saccharomyces cerevisiae PJ69-4A strain cultures were independently transformed with the two bait plasmids and single clones were assayed for protein expression by Western blot using a HESX1 antibody [19].
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Two baiting strategies have been developed for inclusion into the control program, a protection strategy and a control strategy.
We investigated whether the animals were choosing the two baited cups more often than expected by chance (50%) when pooled together for all food and time intervals.
To examine whether the three components (i.e. what, where and when) were structured into a single memory the authors further showed that the animals encoded two baiting events in an integrated fashion [39].
The blend attracted 3 to 5 times more mosquitoes than humans when the two baits were in different experimental huts (10 100 metres apart), but was equally or less attractive than humans when compared side by side within same huts.
To demonstrate that the dynamics of two distinct protein interactions can be monitored in single cells, two bait-PARCs were generated that were fused to bait proteins with distinct response properties: the regulatory subunits RI-α and RII-β.
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CEO of Professional Science Editing for Scientists @ prosciediting.com