Exact(8)
Each treatment was represented by two aquaria.
In each of the two systems, two aquaria can be heated up to 8°C.
After 1 and 6 weeks of Zn post-exposure, all fish from two aquaria from each group were collected, counted, and weighed.
Zinc was added to 24 100-L aquaria to obtain the nominal concentrations of 0.0, 5.0 and 10.0 mg Zn/L and each treatment was represented by 8 aquaria; two aquaria for each period at each Zn concentration.
A vertically sliding opaque panel was initially inserted between the two aquaria so that the experimental male could not see the female located in the second aquarium during 2.5 min. After this time, the sliding opaque panel was raised during the next 2.5 min. The male had then visual access to the female although he could not physically interact with her.
Concerning predator-treatment, further two aquaria (Back aquarium 1, 2) were prepared for back up.
Because there was only one aquarium for 1 ppm nC60 exposure and two aquaria for H2O2 exposures, statistics were not performed on these groups.
The larger trial consisted of fish in two aquaria each of control, 0.5 ppm nC60, and 100 μM hydrogen peroxide (positive control for oxidative damage) and fish in one aquarium of 1 ppm nC60.
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