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For two additional generations, the Times remained rock-ribbed conservative.
Mice were backcrossed at least two additional generations with FVB/N mice for uniformity and genotyped as described (MMHCC and [8]).
All beetle populations, including those from the coevolution treatment, were therefore kept under parasite-free conditions for two additional generations.
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Here we report the results of five additional generations of laboratory evolution starting from 3-2G7.
After the 1,000 historical generations, six additional generations, numbered 1 to 6, were simulated.
The line was then re-derived at the Jackson Laboratory (Bar Harbor, ME), backcrossed onto C57BL/6J for at least six additional generations, and is now maintained on a B6 genetic background.
Seed colour variants were grown for three additional generations to confirm trait stability.
Three additional generations (1001 to 1003) were simulated in which no mutations occurred.
This population was derived from intermating the progenitor population, the IBM Syn10, for four additional generations.
They simulated four additional generations of random selection in order to extend their data set.
Mice were crossed to C57BL/6N (Charles River) for two to five additional generations prior to the experiments described.
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