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All of these analyses were conducted twice per sample in the Laboratory of Aquatic Animal nutrition of Kagoshima University (Japan).
Analyses were performed twice per sample, using a dyereversal procedure in which cDNA from the control was labeled with Cy3 and cDNA from Bb induced was labeled with Cy5.
Procedures were performed twice per sample.
Genotyping was performed at least twice per sample.
The DNA samples were amplified twice per sample using GenomiPhi™ DNA Amplification Kit (Amersham Biosciences).
The mRNA purification was performed twice per sample to ensure the removal of any DNA contamination as well as to reduce the amount of rRNA available in the sample.
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Stimuli were played out 16 times, twice per sampling interval, during the "on" segment of each block.
In this respect, the l1-minimization algorithm was run twice per test sample: the first time using the training data from the same class to construct W w,ij and the second time using the training data from the other classes to construct W b,ij.
All reactions were replicated twice per biological sample.
Minimum and maximum depths were set to five and twice the average depth per sample, respectively.
For the sake of technical replication, all 12 pooled samples were measured twice by preparing two microarrays per sample.
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