Sentence examples for twice fixed from inspiring English sources

After intermittent exposure to sham MF or MF for 5 days, cells were harvested, washed twice, fixed with 4% glutaraldehyde for 2 h, post-fixed with 1% OsO4, dehydrated in graded concentrations of ethanol, and then embedded in resin SPI-Pon 812 (Shell Chemical, Yuhuan, China).

"While bonuses worth more than twice fixed pay would be banned, special treatment would be given to loss-absorbing securities such as shares and debt that can be written down during a crisis, according to E.U. officials.

An EBA cap, which was not legally binding but which the watchdog expected to be adhered to, came into effect in October and limited bonuses to no more than twice fixed salary.

For measurements, the treated cells were washed with PBS twice, fixed in 4% paraformaldehyde for 20 min, and washed twice with PBS for 5 min each, at room temperature.

Cells were then washed twice, fixed with 1% paraformaldehyde, and analyzed with a FACSCalibur® or FACSAria® flow cytometer.

Cells were incubated for 1 hr in the dark at room temperature and then washed twice, fixed, and mounted using ultimate mounting medium (UMM) [46].

Do funny things such as dropping your pencil twice, fixing your hair, Get a future.

After being stained for 30 min, cells were washed twice and fixed in PBS containing 1% paraformaldehyde.

Cells were washed with PBS twice and fixed with 4% paraformaldehyde in PBS for 15 min at 37°C.

For immunocytochemistry, coverslips plated with cells were washed with PBS twice and fixed with 4% PFA at room temperature for 20 min.

The cells were washed with LLB twice and fixed.