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Cells were washed twice, analyzed by immunofluorescence microscopy and subsequently detached, fixed in 2% formaldehyde and analyzed by flow cytometry.
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Thereafter, cells were washed twice and analyzed by FCM.
Cells were washed twice, and analyzed using a FACS Scan flow cytometer (Becton Dickinson).
Cells were stained with an APC-conjugated anti-CD11c antibody (BD biosciences) and a PerCP-conjugated anti-B220 antibody (BD biosciences), washed twice and analyzed by flow cytometry on a FACSCalibur (BD biosciences, Franklin Lakes, NJ).
Cells were washed twice and analyzed with Guava EasyCyte flow cytometer.
Cells were washed twice and analyzed with a FACScan (BD Biosciences, Mountain View, CA).
The cells were again washed twice, then analyzed on a FACSCalibur flow cytometer.
Cells were washed twice and analyzed using a FACScan (BD, Mountain View, CA).
Cells were washed in PBS twice and analyzed for Δψm using FACS.
MEFs were then washed with PBS twice and analyzed by flow cytometry as described above.
The cells were again washed twice, then analyzed on a FACSCalibur flow cytometer (BD Biosciences, UT Southwestern Flow Cytometry Core Facility).
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