Twenty min after starting the i.v. continuous infusions of methoxamine, baseline values of diastolic blood pressure and heart rate were significantly (P < 0.05) increased in all animals (i.e. 140 ± 4 mmHg and 273 ± 4 beats per min, respectively).
Twenty min later, the values of diastolic blood pressure and heart rate were determined again, and then, the vasodepressor responses elicited by i.v. bolus injections of exogenous α-CGRP (0.1, 0.18, 0.31, 0.56 and 1 μg/kg) were examined during the infusions of methoxamine and DHE.
Twenty min after the incubation, miR-328-transfected cells started to detach, while vector-transfected cells remained undetached after 25 min of incubation (Figure 2A).
Twenty min later, treadmill speed was increased at a rate of 1 m/min every 2 min until mice were exhausted.
After twenty min of formalin injection, the second phase (inflammatory) was recorded.
Twenty min pretreatment with 20 μM significantly reduced CPA-evoked intracellular Ca2+ release and SOCE and 6(d)).
Twenty min later, mice were sacrificed and the distribution of fluorescence in the stomach and 10 equal segments of the small intestine was measured.
