Exact(1)
The TψC arm and variable loop are replaced by a single TV loop.
Similar(59)
With the exception of trnC, trnV and trnS1, all tRNAs have T-arm variable loops (TV replacement loops) instead of the T-arm.
(v) TV-loop tRNAs.
Previous reconstructions for both species [ 12] have a D-arm and lack a T-arm (TV-loop).
In D. farinae, tRNA-Ser2 (TCN) was also inferred by tRNAscan-SE (TV-loop) and ARWEN (cloverleaf).
tRNAscan-SE finds a structure only in D. farinae, and this a low-score TV-loop tRNA-Met (0.55).
If the sequence corresponding to the D. pteronyssinus tRNA-Thr is entered into ARWEN, nearly the same TV-loop tRNA is recovered (Fig. 2).
Since we expect tRNA-Ile to be a TV-loop, we constrained the D. farinae sequence to the tRNA-Ile of D. pteronyssinus (Fig. 2).
Based on comparison with D. pteronyssinus and high MFEs (positive for the TV-loop and -1.65 for the cloverleaf), these reconstructions seem unlikely.
We consider these TV-loop structures, having acceptable thermodynamic parameters (Additional file 5, 16) and one compensatory mutation in the acceptor stem, as most probable for Dermatophagoides.
Dermatophagoides retains the presumed ancestral chelicerate gene order "Asp- ATP8- ATP6- COX3-Gly", and this TV-loop tRNA is part of it.
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