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Studies on collagen turnover performed in humans by means of microdialysis techniques show that, after different types of exercise, both synthesis and degradation of collagen are increased, but collagen synthesis prevails and persists longer than collagen degradation [ 119- 121].
We report in this study a prospective 2-year study of a cohort of type 2 diabetic patients with mild nonproliferative DR (NPDR) analyzed using automated images analysis for MA turnover performed in color fundus photographs.
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In total 30 measurements of muscle protein turnover were performed employing a 3-pool model technique with a constant infusion of d5-phenylalanine, leg blood flow measurements, and biopsies to determine tissue enrichments.
To investigate how binding of Dkk1 to LRP6 influences receptor trafficking and turnover, we performed co-transfection of Myc-tagged LRP6 cDNA with human Dkk1 into HEK293 cells.
Subsequent posterior retinal turnover was performed to remove subretinal membranes if present, with direct visualization.
Thus, to reduce the confounding effects of APP-CTFs' turnover, we performed a parallel experiment in which cells were treated with the γ-secretase inhibitor (GSI) compound-E.
Much of this directed protein turnover is performed by proteases that require ATP and the Clp protease is one of the best characterized to date [ 33].
Moreover, to determine whether the accumulation of LC3II induced by PI in MCF7 cells is caused by stimulation of autophagy or inhibition of autophagic vesicle turnover, we performed both morphological and biochemical analysis.
Turnover experiments performed with l-[U-C]-tyrosine and S-adenosyl l-[methyl-C]-methionine showed the concurrent consumption of these molecules, and ∼1 equiv of 4-methyl-5- β-hydroxyethyl) thiazole phosphate was formed per mole of ThiGH.
The levels of Pi as well as other metabolic markers potentially related to cancer are also altered in metabolic bone disease [ 26- 28], so that additional adjustment for alkaline phosphatase, a marker of bone turnover, was performed.
A very detailed and comprehensive analysis regarding immunological processes, cytokines involved in bone resorption and bone turnover was performed in vitamin D receptor (VDR) knockout mice with spontaneously developing arthritis [ 20].
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