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Bone histomorphometry was performed on the proximal tibiae and tibial shafts for bone turnover parameters at week 40.
However, micro-CT does not allow the assessment of bone turnover parameters such as bone formation rate and osteoclast (OC) number and surface.
There were no differences between the BF groups in any of the measured bone turnover parameters (Table 3).
In vivo cell turnover parameters were estimated by intravenous injection of 5-bromo-2'-deoxyuridine (BrdU) and carboxyfluorescein diacetate succinimidyl ester (CFSE).
For efficient optimization, it is also important to choose reasonable starting estimates for the turnover parameters.
In this model, the protein turnover parameters represent the process of dilution due to cell growth.
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Milan et al. added new enzyme turnover parameter to avoid metabolism overflow in GEM of L.lactis[ 65] based on flux balance analysis with molecular crowding [ 66].
However, to obtain 100% turnover, both parameters had to be increased to their maximum coded levels.
It was observed that the estimate for the turnover rate parameter (kout) in the clinical model (0.099) was fairly similar to the estimate for the preclinical model (0.143), suggesting that rates of integrin inhibition are comparable, and only slightly faster in the preclinical setting.
We also investigated the relationship of bone mineral density with various biomarkers of bone turnover and clinical parameters.
To make these investigations objective and measurable, cellular turnover or morphometric parameters such as villus height, crypt depth, and enterocyte height are frequently used [ 1].
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