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The authors determined the analytical closed form of the mean travel time for both the single-command (SC) and the dual-command (DC) cycles varying the warehouse shape factor and the ABC turnover curve.
The significant increase in CSF production from 3 to 12 months and its subsequent decrease to 30 months highly influenced the shape of the turnover curve.
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Turnover curves were followed by measuring the increase in Cy5 fluorescence (640/670 nm) using a Biotek plated reader.
We have performed a sensitivity analysis of all the growth model parameters and found that the qualitative shapes of the mass and turnover curves are preserved.
The rates of increase in Cy5 fluorescence (which is indicative of probe cleavage by cathepsins) were measured for each concentration (a serial dilution starting from a final concentration of 10 μM to 0 μM in triplicate) of quenched substrate from the linear portion of the turnover curves.
The constant turnover, steep learning curve, and pressure to produce make for a "very intense, very specialised environment," maintains Geoff Barton, who leads a research group at the EBI.
Duplicate samples from duplicate cultures were dark acclimated for >15 mins, after which single turnover fluorescence induction curves were measured over a range of background light levels.
Single turnover fluorescence induction curves were measured using a Background Irradiance Gradient – Single Turnover fluorometer (BIG-STf) to measure the photosynthetic conversion efficiency (Fv/Fm) and functional absorption cross section (σPSII) of photosystem II (PSII) as a function of background light intensity as previously described [106].
Finally, despite the very high temporal turnover observed, the dominance diversity curves for 1966 and 1996 were identical (Fig. 3), suggesting that temporal turnover may promote coexistence by avoiding competitive exclusion by the most dominant species.
As one can see in Figure 7, under multiple-turnover conditions the progress curve of MtATP-PRT displays burst kinetics.
Hence direct comparison between F-actin lifetime distribution data from speckle turnover analysis and the decay curve in FRAP experiments may not be valid.
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