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Confocal microscopy analysis revealed that the thickness of biofilms under turnover conditions and non-turnover conditions was approximately 35 and 3.5 μm, respectively.
G. sulfurreducens biofilms were grown in minimal medium with acetate as electron donor (turnover conditions), followed by 24 h incubation in electron donor-depleted medium (non-turnover conditions).
Probing the enzyme under turnover conditions showed mechanistic insights into proton translocation coupled to electron transfer.
Further, the protein binds Zn II) and Co II) in the expected manner and shows ferroxidase activity under single turnover conditions.
In this study, we made quantitative measurements of the DNA glycosylase activity of Pa-AGOG wild type and some engineered variants under single turnover conditions.
After 18 days of continuous measurement in a chronoamperometry experiment under turnover conditions in the absence of O2, the electrode still maintained 75% of the initial current (See Supplementary Fig. 9).
Similar(9)
How PT is precisely coupled and synchronized with electron transfer between [2Fe]H and [4Fe]H remains to be further elucidated, possibly by a time-resolved characterization of the catalytic mechanism under sub-turnover conditions.
Both cloned and synthetic RNA oligonucleotide versions of ribozymes and targets were tested under multiple-turnover conditions using oligonucleotide RNA targets.
The ability of each SOFA-HDV ribozyme to cleave 5'-radiolabeled 575-nt HCV RNA was tested under single-turnover conditions ([Rz]≫[S]).
Rate constant, k f, was evaluated under single-turnover conditions.
Two different effects were observed under multiple-turnover conditions.
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