In this study, the combination of metabolic profiling and turnover analysis (collectively referred to as dynamic metabolic profiling) was applied to explore the effects of stress associated with combined light intensity and nitrogen depletion on carbon metabolism in Chlamydomonas sp. JSC4.
The data obtained from dynamic metabolic profiling (that is, metabolic profiling and turnover analysis) provided valuable information that increases our understanding of metabolic flow in Chlamydomonas sp. JSC4 when cultured in the absence of nitrate and with light of suitable intensity.
These values are similar to the rate constants determined from single turnover analysis in a stopped-flow instrument, as described above, and reflect the decay of the M*ADP·Pi complex in both cases.
For turnover analysis, mice were injected with 2 mg/ml of BrdU i.p. and then maintained on daily-prepared drinking water containing BrdU at 0.8 mg/ml, as previously described [48].
Nagao H, Nishizawa H, Bamba T, Nakayama Y, Isozumi N, Nagamori S, Kanai Y, Tanaka Y, Kita S, Fukuda S, Funahashi T, Maeda N, Fukusaki E, Shimomura I. Increased Dynamics of Tricarboxylic Acid Cycle and Glutamate Synthesis in Obese Adipose Tissue: IN VIVO METABOLIC TURNOVER ANALYSIS.
Huh, K. H. & Wenthold, R. J. Turnover analysis of glutamate receptors identifies a rapidly degraded pool of the N-methyl-d-aspartate receptor subunit, NR1, in cultured cerebellar granule cells.
Extending the zeta diversity framework from a platform of spatial turnover analysis to temporal turnover investigation is an ongoing research area.
For turnover analysis, we counted as new protrusions all new structures (spines or filopodia) appearing between two observations (5 h or 24 h) and characterized by a length of >0.4 µm.
This novel method of turnover analysis does, however, have several limitations.
We readdressed this measurement using single turnover analysis, as this approach is less sensitive to uncertainties in the active protein concentration.
