This allows the SAM output of alignment tools such as NOVOALIGN (http://www.novocraft.com) or BWA-MEM (http://arxiv.org/abs/1303.3997) to be piped directly into SAMBLASTER, which marks duplicates and outputs read-id grouped SAM, which in turn is piped to SAMTOOLS or SAMBAMBA for sorting and/or compression, without the need to store any intermediate files.
And though it's been given the Merlin treatment (stalls selling toy swords at every turn and piped medieval music in the garden) the castle interior itself is still gobsmackingly impressive.
"To be gnawed out of our graves, to have our skulls made drinking-bowls, and our bones turned into pipes to delight and sport our enemies, are tragical abominations".
The Leeds family has been growing wine grapes at its Rutherford ranch since before the Great Depression and has never turned to pipes and hoses.
We simply need to turn the pipe around and put the water and an ocean's worth of nutrients to good use back on land, where we need it.
The Iranian-born, French-raised musician soundtracks the video with a cover of the 1993 hit, turning those pipes into bewitching hums and drawing the entire song out over 10 minutes, set to imagery of semi-erotic, semi-awkward encounters between couples, and an eerie scene of someone climbing out of their own grave.
At the same time, slowly turn the pipe wrench that is connected to the faucet counterclockwise until the faucet is loosened.
A labor dispute between the league and its regular officials, however, turned those pipe dreams into reality.
(I didn't mind the clownish cowboy or the stereotyped Europeans; I even laughed at the exaggerated French accent that turned "peace pipe" into another, less savory item.
"You are the person who opens the doors to the furnace, drops the color onto the bubble, and shields the gaffer from the heat," Biddle said, "or you are the person who gathers the glass once it gets to a certain size and turns the pipe when it gets heavy, and places the piece in the annealing oven when it is finished".
