Turbidity test of water samples was measured using 2100P (HACH) turbidity meter and compared before and after treatment (Amagloh and Benang 2009).
Specifically, within 5 minutes the Con A-nanosensors were able to determine that E. coli had an ampicillin MIC of 8 µg (Figure 7A), in line with the data from the dextran-coated nanosensors in the competition assay or the turbidity test.
The turbidity test was performed according to the report.
As for C. albicans, a simple turbidity test [ 8] was used to determine the MIC value of menthol.
Other methods, such as refractometry, zinc sulfite turbidity test, sodium sulfate turbidity test, serum γ-glutamyl transferase activity, whole blood glutaraldehyde coagulation test, and ELISA have been used to identify calves with FTPI with varying degrees of accuracy [ 2, 12, 13].
Assays can be divided into semiquantitative (the Berry spot and Ames spot tests) and quantitative (the carbazole reaction, cetylpyridinium turbidity test, alcian blue reaction, dimethylmethylene blue test, and azure A and B method).
Among the quantitative assays, the carbazole reaction has a low sensitivity for MPS IV (Morquio syndrome) and the cetylpyridinium turbidity test has a low sensitivity for MPS IV and MPS III (Sanfilippo syndrome); therefore, these assays should be avoided [ 5, 35].
The time needed for the turbidity of each tested sample to exceed OD650 nm at 0.1 is referred to as the threshold time (Tt) [ 19].
The data of OD600 nm in the turbidity reduction test at different pHs and temperatures were analyzed by a Student's t test and with a p value of <0.05 considered as significant.
